| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Bacillus subtilis | AGTAGCGCACGTGCAAGT | ACCATGCCGAATCCCTTCA | 59.97 | 59.00 | 261 | 88 |
100.00%
|
100.00%
|
| Primer 32 | Bacillus subtilis | AGTAGCGCACGTGCAAGT | GACCATGCCGAATCCCTTCA | 59.97 | 60.11 | 262 | 88 |
100.00%
|
100.00%
|
| Primer 33 | Bacillus subtilis | AAACGTGCAGCACCTGTG | TCAGCACGGCTTCAAGCA | 58.89 | 59.89 | 177 | 88 |
100.00%
|
100.00%
|
| Primer 34 | Bacillus subtilis | CGTTTCGCCAACTGGAACG | AATGCAGACCGAGCAGCA | 60.08 | 59.65 | 169 | 88 |
100.00%
|
100.00%
|
| Primer 35 | Bacillus subtilis | CGCACCTCCGGAAGATTCA | TTGACGCTTTCCCGTGCA | 59.78 | 60.20 | 190 | 88 |
100.00%
|
50.00%
|
| Primer 36 | Bacillus subtilis | GAGGAGTGCTGCAGACTCA | TGACAGGCAAACACGGCA | 59.03 | 60.12 | 202 | 88 |
100.00%
|
100.00%
|
| Primer 37 | Bacillus subtilis | ACAGCAGAACGCACGAGT | AACAGAAGCGCTCCGTCTC | 59.58 | 60.08 | 222 | 87 |
100.00%
|
100.00%
|
| Primer 38 | Bacillus subtilis | ACAGCAGAACGCACGAGT | TCCCCTTTGGCACGTTAGC | 59.58 | 60.30 | 263 | 87 |
100.00%
|
100.00%
|
| Primer 39 | Bacillus subtilis | TGCTCCAAGCGCTGGTAAA | CAAAAGCGGTTGCCAGCA | 59.93 | 59.58 | 198 | 87 |
100.00%
|
100.00%
|
| Primer 40 | Bacillus subtilis | GTTTCGCCAACTGGAACGG | AATGCAGACCGAGCAGCA | 59.72 | 59.65 | 168 | 86 |
100.00%
|
100.00%
|